Primer pozne smrti po zdravljenju meduloblastoma

A 27-year-old male was treated for medulloblastoma with complete removal of tumor, postoperative RT to CNA with 36 Gy and boost to the posterior fossa 16 Gy. Nine years later, a suprasellar recurrence was treated with Carboplatin and Vepezid and 33 Gy to the tumor bed. Three years later he complained about headaches, periodic loss of conciousness and progressively showed bizarre behavior. He was referred to psychiatrist. No recurrence was found. He did not appear on the day of follow-up appointment. He died at home, at the age of 43 years, 16 years after the first treatment. The cause of death determined by forensic autopsy : anemic subacute infarction in the left cerebellum.Ni abstrakta

A Case of Late Death after Treatment for Medulloblastoma

A 27-year-old male was treated for medulloblastoma with complete removal of tumor, postoperative RT to CNA with 36 Gy and boost to the posterior fossa 16 Gy. Nine years later, a suprasellar recurrence was treated with Carboplatin and Vepezid and 33 Gy to the tumor bed. Three years later he complained about headaches, periodic loss of conciousness and progressively showed bizarre behavior. He was referred to psychiatrist. No recurrence was found. He did not appear on the day of follow-up appointment. He died at home, at the age of 43 years, 16 years after the first treatment. The cause of death determined by forensic autopsy : anemic subacute infarction in the left cerebellu

Molecular genetic identification of skeletal remains from the Second World War Konfin I mass grave in Slovenia

This paper describes molecular genetic identification of one third of the skeletal remains of 88 victims of postwar (June 1945) killings found in the Konfin I mass grave in Slovenia. Living relatives were traced for 36 victims. We analyzed 84 right femurs and compared their genetic profiles to the genetic material of living relatives. We cleaned the bones, removed surface contamination, and ground the bones into powder. Prior to DNA isolation using Biorobot EZ1 (Qiagen), the powder was decalcified. The nuclear DNA of the samples was quantified using the real-time polymerase chain reaction method. We extracted 0.8 to 100 ng DNA/g of bone powder from 82 bones. Autosomal genetic profiles and Y-chromosome haplotypes were obtained from 98% of the bones, and mitochondrial DNA (mtDNA) haplotypes from 95% of the bones for the HVI region and from 98% of the bones for the HVII region. Genetic profiles of the nuclear and mtDNA were determined for reference persons. For traceability in the event of contamination, we created an elimination database including genetic profiles of the nuclear and mtDNA of all persons that had been in contact with the skeletal remains. When comparing genetic profiles, we matched 28 of the 84 bones analyzed with living relatives (brothers, sisters, sons, daughters, nephews, or cousins). The statistical analyses showed a high confidence of correct identification for all 28 victims in the Konfin I mass grave (posterior probability ranged from 99.9% to more than 99.999999%)

Identifikacija voznikov v prometnih nesrečah in določanje pozicij udeležencev v vozilu po prstnih sledeh

The following paper aims to illustrate certain investigative activities in the forensic analysis and examination of the scene of traffic accidents. When a traffic accident occurs, the scene must be secured as soon as possible to enable professional and proper forensic investigation. Failure to secure the accident scene might result in losing or contaminating the traces, which makes it more difficult to prove or explain trace evidence in further procedure or even makes such evidence inadmissible. The topic is discussed from the viewpoint of crime scene examination, since analysing and investigating traffic accidents requires a great deal of expertise and attention of the investigators. Complex traffic accidents include feigned accidents, hit-and-run accidents as well as accidents in which the driver and passengers, dead or alive, need to be identified. In identifying the passengers, standard criminal investigation methods as well as police forensic and forensic medicine methods are followed. Such methods include confirming the identities with identity documents, other documents and vehicle ownership, fingerprints, biological traces, fibre traces, contact traces, traces of physical injuries on the driver and passengers, etc. According to the results obtained in fingerprint detection on human skin surfaces, this method can also be applied in confirming physical contact between the driver and the passengers in the accident, e.g. in the event of moving the victims and changing the scene of the accident.Namen prispevka je pojasniti določena preiskovalna dejanja pri analizi t.j. pri kriminalistično tehničnem oz. forenzičnem ogledu kraja prometne nesreče. V primeru prometne nesreče je treba za strokovno in pravilno forenzično preiskovanje čim hitreje zavarovati kraj. Če se kraj dejanja ne zavaruje, se sledi izgube ali kontaminirajo, kar vodi do težjega dokazovanja ali pojasnjevanja dokazov v postopku ali celo do njihove popolne nezmožnosti. Vidik obravnavanja je usmerjen v izhodišča ogledne dejavnosti, saj analiza prometnih nesreč v cestnem prometu in preiskovanje zahtevajo od preiskovalcev veliko znanja in pozornosti. Zelo zahtevni so primeri fingiranih prometnih nesreč, prometnih nesreč ko vozniki ali udeleženci zapustijo kraj dogodka ter nesreče, kjer je treba ugotoviti identiteto voznika in udeležencev, živih ali mrtvih. Ugotavljanje istovetnosti udeležencev poteka po ustaljenih kriminalističnih oz. forenzičnih ter sodnomedicinskih metodah. Mednje se prišteva tudi potrditev identitete po osebnih dokumentih, dokumentih in lastništvu vozil, prstnih sledeh, bioloških sledeh, sledeh vlaken in kontaktnih sledeh, sledeh telesnih poškodb voznika in udeležencev ip. Rezultati preiskave detektiranja prstnih sledi na površini človeške kože so pokazali, da je tudi tovrstno metodo možno uporabiti pri potrditvi fizičnega kontakta voznika z udeleženci v prometni nesreči, npr. pri premikanju žrtev in fingiranju kraja dejanja

Slovenian population data for five new European Standard Set short tandem repeat loci and SE33 locus

Aim To establish the allele distribution and statistical parameters of forensic interest for the D10S1248, D22S1045, D2S441, D1S1656, D12S391, and SE33 loci in Slovenian population and to compare allele frequencies with those from other populations. Methods We analyzed blood and buccal swab samples from 333 unrelated, healthy Slovenian individuals. All samples were genotyped using the AmpFlSTR NGM Kit to obtain the allele frequency data for the loci D10S1248, D22S1045, D2S441, D1S1656, and D12S391. Samples from 113 individuals were also analyzed using the PowerPlex ESX 17 system to obtain the allele frequency data for the SE33 locus. Allele frequencies and statistical parameters of forensic interest were determined and frequency profiles compared between Slovenian and other European Caucasian populations using the Arlequin software, version 3.5.1.3. Results The investigated short tandem repeat (STR) loci in Slovenian population had a great discriminating potential with a combined discrimination power of 0.99999998. The highest discrimination power and polymorphism information content were observed for the SE33 locus, followed by loci D1S1656, D12S391, D10S1248, D2S441, and D22S1045. When Slovenian allele frequency distribution was compared with other European populations, deviations were found only for Spanish and Italian population for D2S441 and D12S391. Conclusion Slovenian population does not differ significantly from other European populations in terms of allele frequency distributions for the six analyzed STR loci. Based on forensic efficiency values, SE33 may be considered the most informative locus, which makes it especially useful in forensic investigations

Searching for the mother missed since the Second World War

The aim of the study was to perform the genetic identification of a human cranium from a Second World War gravesite in Slovenia and find out if it belonged to the mother of a woman used as a family reference. Both genetic and anthropological examinations were carried out. The genetic examination was performed on 2 molars and petrous bone. Prior to DNA isolation 0.5 g of tooth and bone powder was decalcified. The DNA was purified in a Biorobot EZ1 (Qiagen) device. The nuclear DNA of the samples was quantified and short tandem repeat (STR) typing performed using two different autosomal and Y-STR kits. Up to 22.4 ng DNA/g of powder was obtained from samples analyzed. We managed to obtain nuclear DNA for successful STR typing from the left second molar and from the petrous bone. Full autosomal genetic profile including amelogenin locus revealed the male origin of the cranium that was further confirmed by the analyses of Y-STRs. The same conclusions were adopted after the anthropological analysis which identified the cranium as that of a very young Caucasoid male. The male origin of the cranium rejected the possibility of motherhood for the compared daughter. For traceability in the event of contamination, we created an elimination database including genetic profiles of the nuclear and Y-STRs of all persons that had been in contact with the analyzed cranium and no match was found